N4 Pharma Share Discussion Threads

[featherby81] 17/8/2018 10:42

Future and existing investors would do well to remember N4 have applied equally as much attention to the patents as they have the products.

[dplewis1] 17/8/2018 10:04

Not involving N4 but one of the companies that they hope to emulate released this yesterday:https://www.cambridgenetwork.co.uk/news/crescendo-biologics-technical-milestone-collaboration-takeda/Shame it's privately held but at least it's evidence these deals do materialise (although worth noting they have raised over $100mm in various funding rounds).

[dplewis1] 17/8/2018 08:26

And lo the case for Nuvec is proven. Let me increase my holding post-haste and wait for those contracts to roll in. When lambo?

[hughwilson] 16/8/2018 23:25

With regards to the issues Nobbo raised. I don`t have to prove anything because all the issues raised have been covered within presentations and/or RNS news releases. Anyone paying attention would have known the answers already. The fact that Nobbo is even raising these issues only goes to show that he has done no research and has very little knowledge about N4 or Nuvec. I would suggest that he does some research and gets up to speed.

[dplewis1] 16/8/2018 18:26

I'd have a guess that Hugh was never invited onto the school debating team given responses like that. Surely if nobby is so ill-informed this is a golden opportunity to dissect the points he made and maybe even win an argument for the first time. As jimbo said earlier, it just shows a reluctance to discuss matters in detail - and that's from the self proclaimed expert. That can only really lead to one conclusion..

[ih_575893] 16/8/2018 17:51

I think you two should just meet up and have a snog. Sort it out FFS.
Neither of you are proving anything. All looks very knowledgeable on paper but where’s your source. Source info please and you’re probably get more respect.

Just looks like a load of google copy and paste.

[hughwilson] 16/8/2018 17:24

Nobbo, once again you have proven that you have done zero research and are ill-informed because most of what you are talking about has already been successfully completed by Nuvec.
You really know very little about N4 or Nuvec do you.

[saint in exile] 16/8/2018 15:41

Makes me laugh the keyboard chemists that know more that the actual chemists!

[onzima] 16/8/2018 11:53

".... experimentally this is not simple at all to get right."

If it was, everyone would already have done it.

[jimbobaroony] 16/8/2018 10:35

Nobby, I personally have no idea if you are talking pharma sense or not. However, I notice Hugh is reluctant to talk technicals with you, which is interesting. He is supposed to be the resident expert.

[nobbygnome] 16/8/2018 07:23

So if you think about what has to happen to get the end result here, it really is quite complicated. The particles first of all need to get to the target cells whatever they are. There is no specific targeting so that is just a random process. I presume essentially this only happens at the injection site because otherwise there wouldn't be enough particles to go round every cell in the body!

Once the particle is bound to the cell it needs to be internalised in a pinocytotic vesicle according to Nigel. I have my doubts about that because that process is to do with the presentation of protein antigens to the immune system. Assuming this is true, the DNA or RNA somehow has to get out of the vesicle into the cytoplasm intact. This is a little surprising because the vesicle is a hostile environment full of various enzymes including nucleases.

Once in the cytoplasm the DNA needs to be transcribed and then translated and RNA just translated. The resultant protein needs to be excreted and reach a high enough level in the blood to stimulate an immune response. The immune system tends not to respond to proteins at very low (or very high) concentrations. So as the protein will be diluted body wide, a lot of it needs to be produced so it is is critical how many cells get transfected.

So really not an easy process at all and explains why I say that experimentally this is not simple at all to get right.

[nobbygnome] 15/8/2018 21:56

Wherever the spikes are😜

[featherby81] 15/8/2018 21:32

Do you mean loaded onto the spikes on the outside?

[nobbygnome] 15/8/2018 21:26

>> featherby

When I say production of the particles, I mean with the RNA/DNA inside not the particles themselves. I suspect that process will have to be tweaked in each individual case although there will be some basic conditions.

[featherby81] 15/8/2018 21:00

Thanks for the post Nobby, we appreciate your time used for researching Nuvec. Given the concept is still at a research state and clearly the particles are currently produced without concern or the Japanese trip with N4 offering participants to use the particles wouldn’t have happened. The proof of concept requirement was a stipulation to receiving this invitation unless I’m wrong.

[dplewis1] 15/8/2018 20:45

You've had a busy day and are tired but you find the energy to be rude..and also to reply to some of nobby's other posts. Why don't you actually respond to the points that nobby made with your analysis and maybe there's a decent discussion? Maybe you don't want to show yourself up? As for my posting history, as you have seen I signed up here around 18 months ago. I have said before that prior to that I was an equity trader on an algo desk for many years so would not have looked at advfn. However I now no longer work in a front office role due to the impact a 12 hour+ day had on my family life, so I have the benefit of a more senior role with much more reasonable hours and less of a compliance oversight. Were there lots of people booking your taxi today and asking for a ride to dreamlands in Margate?

[nobbygnome] 15/8/2018 20:43

PS I know nothing about oilers or mining companies.....

[nobbygnome] 15/8/2018 20:40

Thank you! That post proves i said I don't know everything...... I assume you can read....

[hughwilson] 15/8/2018 20:38

No time Nobbo, just too busy now.

[hughwilson] 15/8/2018 20:37

No time for it Nobbo but regarding-

"I don't think I ever said I knew everything. You are making stuff up again...."

You keep saying that I make things up.

Nobbygnome - 15 Jul 2018 - 08:29:36 - 244 of 733

" I know a lot of you guys on here hate to contemplate that you may actually be conversing with an expert in the field but that is the reality. That is not me being arrogant just me stating the truth. And if there is some aspect of drug development I don't know about, I have a network of colleagues around the world who I can ask"

I am amazed you can squeeze through the door, given the size of your ego.

[nobbygnome] 15/8/2018 20:30

If you want, I can go through all the inaccuracies in the latest presentation. I don't think Nigel knows what pinocytosis is....

[hughwilson] 15/8/2018 20:21

Anyway, I am tired and I have things to do before work tomorrow so another time.

[nobbygnome] 15/8/2018 20:20

What hugh doesn't realise is all the things which can go wrong when you are doing the sort of experiments we are talking about here. I know because I have actually been there and done it. Twenty years at the bench followed by 14 years developing, launching, marketing and selling a billion pound drug. Hmmmm I think I am qualified to comment.....although I don't think I ever said I knew everything. You are making stuff up again....